SimpleMicrobiome

SimpleMicrobiome supports microbiome data visualization, diversity analysis, differential abundance, and network analysis.

Start here: Go to the Data Loading menu first.
QZA Converter (External Service): Open Converter Tool

Quick Workflow

  1. Data Loading: Upload feature table, taxonomy, and metadata.
  2. Preprocessing: Filter data by read counts and user-selected options.
  3. Analysis: Run visualization/diversity/DA/network modules as needed.

Version History

Show updates
  • 260415: QZA Converter Tool added.
  • 260414: Network Comparison function added.
  • 260408: Network Analysis modules added.
  • 260407: Random Forest module added.
  • 260406: MaAsLin2 module added.
  • 260321: Taxa Comparison feature added.
  • 260310: Preprocessing functionality improved.
  • 251210: First public version released.
Kangwon National University © 2025-2026 SimpleMicrobiome | Microbial Genomics Lab
Contact: yongjoon (at) kangwon.ac.kr

Data Input

Download Example

This tool requires 3 files:

  • ASV_table.tsv from table.qza
  • taxonomy_table.tsv from taxonomy.qza
  • metadata.tsv prepared manually by the user

To convert QZA files to TSV, use QZA Converter

Sample Selection Overview

Select samples by minimum read count.

Unselect rows in the table to EXCLUDE samples.


                

                

                

                  
                  Group-based Toggle
                

                

                  
                  

                    
                    
                  

                

                

                  
                  

                    
                    
                  

                

                
                

                
                

                
              

            

            

              

                

                  
                  Individual Sample Selection (Click to Toggle)
                

                

                  

                

              

            

          

        

        

          

            

              
            

            

              

            

          

        

        

          

            

              
            

            

              

            

          

        

        

          

            

              
            

            

              

              

              

            

          

        

        

          
          

            

              
            

            

              

                
                

                  
                  

                    

                      
                      NMDS Ordination
                    

                    

                    
                  

                

              

              

              

                
                Statistical Test: PERMANOVA Results
              

              

                

              

              

                
                Clustering Results
              

              

                

              

              

                
                EnvFit Results
              

              

                

              

            

          

        

        

          

            

              
            

            

              

                
                

                  
                  
                  
                

              

            

          

        

        

          

            

              
            

            

              

                
                

                  
                  
                  
                

              

            

          

        

        

          

            

              
            

            

              

                
                

                  
                  
                  
                  
                  
                

              

              

              

                
                Result
              

              

            

          

        

        

          
          

            

              
            

            

              

                
                

                  
                  
                  
                  

                    

                  

                  
                  

                    

                  

                  

                    

                  

                  

                    

                  

                

              

            

          

        

        

          
          

            

              
            

            

              

                
                

                  
                  
                  
                  

                    

                  

                  
                  

                    

                  

                  

                    

                  

                  

                    

                  

                

              

            

          

        

        

          

            

              
              Citation
            

            
References are grouped by module (APA style).